Research paper on column chromatography
From: Justin H.
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This page shows how the same principles used in thin layer chromatography can be applied on a larger scale to separate mixtures in column chromatography. Column chromatography is often used to purify compounds made in the lab. Note: It is important to read the introductory page about thin layer chromatography before you continue with this one - particularly the part about how thin layer chromatography works, although you will also need some idea about how to make a thin layer chromatogram. In thin layer chromatography, the stationary phase is a thin layer of silica gel or alumina on a glass, metal or plastic plate. Column chromatography works on a much larger scale by packing the same materials into a vertical glass column. Various sizes of chromatography columns are used, and if you follow a link at the bottom of the page to the Organic Chemistry section of the Colorado University site, you will find photographs of various columns.
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Experiment 6: Column Chromatography
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In: Science. Paper and Column Chromatography Report Guidelines 1. The reasoning is based primarily off of the paper chromatography experiment. In paper chromatography, the stationary phase is a very uniform absorbent paper. The mobile phase is a suitable liquid solvent or mixture of solvents. But, the stationary phase is more polar because the paper is not actually polar at all, compared to, probably, water as the mobile phase.
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